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Welcome to Laboratory Protocol Development Wiki!

LPDWiki is a project being developed by Christopher Chapman for the opensource development and tracking of experimental procedures.

Labs Currently Using LPDWiki

Seker Lab
General Lab Protocols Cell Culture Protocols Cell Staining Protocols Microfluidic Protocols Electrochemistry Protocols
Biosafety Cabinet Start-up Cell Culture Media Preparation General Staining Protocol Laser Cutting Channel Mold Phosphate Buffer Preparation
Biosafety Cabinet Shutdown Trypsin Preparation Phalloidin Stain (F-actin) Microfluidic Device Fabrication 0.5 M Sodium Bicarbonate and 75 mM Magnesium Chloride Solution Preparation
Biological Material Disposal Culture Passaging GFAP Stain (Astrocyte - Entire cell) Microfluidic Cell Culture DNA Probe and Target Aliquoting
70% EtOH Preparation Primary Neuron-Glia Co-Culture Tubulin-BIII Stain (Neuron - Entire cell) Quantification of DNA using UV - Vis
Incubator Cleaning Paraformaldehyde Fixation Vinculin Stain (Focal Adhesions) DNA Reduction and Immobilization
Water Bath Cleaning Glutaraldehyde Fixation and SEM Preparation of Cells NeuN Stain (Neuron - Cell bodies) Target Hybridization
ImageJ Nestin Stain (Neural Precursor) Solution Preparation: x1 PBS, 20 µM Methylene-Blue, 1 mM MCH
General Macro- and Microfabrication Techniques Nanoporous Gold (np-Au) Fabrication and Preparation Protocols Material Characterization Techniques
Piranha Cleaning np-Au Precursor Alloy Fabrication Scanning Electron Microscopy
Lithography and Photoresists np-Au Dealloying Energy Dispersive X-Ray Spectroscopy (EDS/EDXS)
Reactive Ion Etching Laser Annealing of np-Au Dynamic Speckle Autocorrelation Spectroscopy
Sputter Deposition Fabrication of np-Au Material Libraries Electrochemical Characterization of Surface Area
Evaporation Deposition Plasma Cleaning np-Au Samples

Editing MediaWiki: Getting started

Support for the creation of this site and the protocols contained within has been provided by the National Science Foundation.

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