0.5 M Sodium Bicarbonate and 75 mM Magnesium Chloride Solution Preparation

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Necessary Materials

1. Sodium Bicarbonate Powder, (NaHCO3)

  • MW = 84.01 g/mol

2. Magnesium Chloride, (MgCl2)

  • MW = 203.03 g/mol

3. 25 mM Phosphate Buffer

4. 2 ml tube, 10 ml tube

Procedure

Important:

  • The following formula was used to find required powder weight to make solutions of desired concentration and volume:
Desired Concentration [M] * Desired Volume [ml] * MW [g/mol] = Mass [g]

0.5 M Sodium Bicarbonate Solution, V = 1 ml

1. Weigh 42 mg of Sodium Bicarbonate powder and transfer to 2 ml tube.

  • Weight found using the formula above

2. Add 1 ml of 25 mM PB to the tube.

4. Vortex until all powder is dissolved.

  • Note: not all powder has to dissolve, because this is saturated solution.

5. Label: 0.5 M Sodium Bicarbonate Solution in 25 mM PB. (Initials, Date)

75 mM Magnesium Chloride Solution, V = 5 ml

1. Weigh 76 mg of Magnesium Chloride powder and transfer to 5 ml tube.

  • Weight found using the formula above

2. Add 5 ml of 25 mM PB to the tube.

4. Vortex until all powder is dissolved.

5. Label: 75mM Magnesium Chloride Solution in 25 mM PB. (Initials, Date)